Cryopreserved potato shoot tips showed genotype-specific response to sucrose concentration in rewarming solution (RS)
نویسندگان
چکیده
منابع مشابه
Cryopreservation of Potato Shoot Tips
Potato is one of the most important staple crops in both developed and developing countries of the world. Potato genetic resources are a prerequisite for potato breeding in both conventional and genetic engineering programmes. Cryopreservation has long been considered an ideal means for the long-term conservation of plant genetic resources. This review provides comprehensive information on vari...
متن کاملGene amplification from cryopreserved Arabidopsis thaliana shoot tips.
Cryopreservation is a way to store elite quality plant germplasms. The exact mechanism of stress tolerance during cryopreservation is unknown. Unavavailability of a detailed protocol for understanding the molecular genetics of plant cryostress is a major obstacle in plant cryobiology research. This paper describes the methods of extraction of total RNA from cryogenically stored plant tissues ac...
متن کاملStCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration.
StCDPK1 encodes a calcium-dependent protein kinase (CDPK) from Solanum tuberosum, which is transiently induced upon tuberization in swelling stolons. In situ hybridization determined that StCDPK1 mRNA is localized in the apical dome of tuberizing stolon tips, close to the region where sucrose was reported to accumulate. The expression of StCDPK1, and other tuber-specific genes was enhanced when...
متن کاملPreservation of In Vitro Grown Shoot Tips of Potato (Solanum tuberosum L.) by Different Methods of Cryopreservation
Cryopreservation has been recognized as a practical and efficient tool for long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of sucrose concentration, hardening temperature and different cryopreservation methods on the survival rate of potato shoot tips after cryopreservation. Excised shoot tips of in vitro plantlets of potato cultivars, At...
متن کاملIn Vitro Response of Encapsulated Shoot Tips of Spilanthes Acmella
Multiple shoots were achieved through axillary bud explant of Spilanthes acmella on MS medium supplemented with 0.5mg/l BA. In vitro developed axillary buds and tiny shoots isolated from the multiple shoots were encapsulated in different strength of sodium alginate. The present work explains the role of components of culture medium on morphogenic response of encapsulated shoot tips to various t...
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ژورنال
عنوان ژورنال: Plant Cell, Tissue and Organ Culture (PCTOC)
سال: 2018
ISSN: 0167-6857,1573-5044
DOI: 10.1007/s11240-018-1520-8